Characterizing Transcription Factor Induction using the Monod - Wyman - Changeux
نویسندگان
چکیده
11 Allosteric molecules serve as regulators of cellular activity across all domains of life. We present a general 12 theory of allosteric transcriptional regulation that permits quantitative predictions for how physiological 13 responses are tuned to environmental stimuli. To test the model’s predictive power, we apply it to the 14 specific case of the ubiquitous simple repression motif in bacteria. We measure the fold-change in gene 15 expression at different inducer concentrations in a collection of strains that span a range of repressor 16 copy numbers and operator binding strengths. After inferring the inducer dissociation constants using 17 data from one of these strains, we show the broad reach of the model by predicting the induction profiles 18 of all other strains. Finally, we derive an expression for the free energy of allosteric transcription factors 19 which enables us to collapse the data from all of our experiments onto a single master curve, capturing 20 the diverse phenomenology of the induction profiles. 21
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